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验证数据展示

  • WB analysis of HEK-293 cell using 18544-1-AP (same clone as 18544-1-PBS)

    WB analysis of HEK-293 cell using 18544-1-AP (same clone as 18544-1-PBS)

    WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE). This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 18544-1-AP (same clone as 18544-1-PBS)

    WB analysis of PC-3 using 18544-1-AP (same clone as 18544-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18544-1-AP (same clone as 18544-1-PBS)

    IF Staining of HeLa using 18544-1-AP (same clone as 18544-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX4 antibody (18544-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CBX4 Polyclonal antibody, PBS Only

CBX4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 18544-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

18544-1-PBS targets CBX4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CBX4 fusion protein Ag13425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromobox homolog 4 (Pc class homolog, Drosophila)
别名 Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4
计算分子量 61 kDa
观测分子量 ~75 kDa
GenBank蛋白编号BC014967
基因名称 CBX4
Gene ID (NCBI) 8535
RRIDAB_2878552
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00257
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.