验证数据展示
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11149-1-AP
产品信息
CL650-11149 targets CBP,EIF4E in applications and shows reactivity with human samples.
| 经测试应用 | Application Description |
| 经测试反应性 | human |
| 免疫原 | CBP,EIF4E fusion protein Ag1626 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 4E |
| 别名 | CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein |
| 计算分子量 | 29 kDa |
| 观测分子量 | 26-29 kDa, 50 kDa |
| GenBank蛋白编号 | BC012611 |
| 基因名称 | EIF4E |
| Gene ID (NCBI) | 1977 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06730 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance. This is a rabbit polyclonal antibody raised against the full-length of human EIF4E. This protein can form a dimer (~50 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CBP,EIF4E antibody CL650-11149 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |