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CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Cat No : 11806-1-AP

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Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

验证数据展示

WB analysis of human brain using 11806-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11806-1-AP (CAPZA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 11806-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 11806-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 40x lens).
IP experiment of SH-SY5Y using 11806-1-AP
IP result of anti-CAPZA1 (IP:11806-1-AP, 4ug; Detection:11806-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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Cat No. 11806-1-AP

Size

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经过测试的应用

Positive WB detected in	human brain tissue
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human ovary tumor tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 5 publications below
IP	See 1 publications below

产品信息

11806-1-AP targets CAPZA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	IHC, IP, WB, ELISA
Cited Applications	WB, IP, IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Immunogen	CAPZA1 fusion protein Ag2267 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	capping protein (actin filament) muscle Z-line, alpha 1
Synonyms	CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1
Calculated Molecular Weight	286 aa, 33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC000144
Gene Symbol	CAPZA1
Gene ID (NCBI)	829
RRID	AB_2070037
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P52907
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

实验方案

Product Specific Protocols
WB protocol for CAPZA1 antibody 11806-1-AP	Download protocol
IHC protocol for CAPZA1 antibody 11806-1-AP	Download protocol
IP protocol for CAPZA1 antibody 11806-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IP	J Cell Biol Heterodimeric capping protein is required for stereocilia length and width regulation. Authors - Matthew R Avenarius View Article
human	WB,IHC	Cell Mol Life Sci HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers. Authors - Gemoll Timo T View Article
human	WB	J Exp Clin Cancer Res CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma. Authors - Deng Huang KD Validated View Article
human	IHC	Invest Ophthalmol Vis Sci Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not. Authors - Linge Annett A View Article
human	WB,IHC	Int J Oncol Prognostic value of CAPZA1 overexpression in gastric cancer. Authors - Lee Young-Joon YJ KD Validated View Article
human	WB,IHC	Melanoma Res Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma. Authors - Sun Daxin D View Article

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CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for IHC, IP, WB, ELISA

验证数据展示

WB analysis of human brain using 11806-1-AP

IHC staining of human ovary tumor using 11806-1-AP

IHC staining of human ovary tumor using 11806-1-AP

IP experiment of SH-SY5Y using 11806-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Heterodimeric capping protein is required for stereocilia length and width regulation.

HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.

Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.

Prognostic value of CAPZA1 overexpression in gastric cancer.

Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.