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验证数据展示

  • WB analysis using 28476-1-AP (same clone as 28476-1-PBS)

    WB analysis using 28476-1-AP (same clone as 28476-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28476-1-AP (CAPN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 28476-1-AP (same clone as 28476-1-PBS)

    IHC staining of mouse skeletal muscle using 28476-1-AP (same clone as 28476-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28476-1-AP (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN3 antibody included

CAPN3 Polyclonal antibody, PBS Only

CAPN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28476-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Calpain-3, Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CAPN3 Ready-To-Use IHC Kit. CAPN3 primary antibody included.

产品信息

28476-1-PBS targets CAPN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CAPN3 fusion protein Ag29454 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain 3, (p94)
别名 Calpain-3, Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3
观测分子量 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank蛋白编号BC146672
基因名称 CAPN3
Gene ID (NCBI) 825
RRIDAB_2881150
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20807
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).