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验证数据展示

  • IHC staining of mouse spleen using 12009-1-AP

    IHC staining of mouse spleen using 12009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 12009-1-AP

    IHC staining of human tonsillitis using 12009-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 12009-1-AP

    IHC staining of human tonsillitis using 12009-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 12009-1-AP

    WB analysis using 12009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12009-1-AP (CAMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12009-1-AP

    IHC staining of mouse brain using 12009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12009-1-AP

    IF Staining of HeLa using 12009-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAMP antibody (12009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human tonsillitis using 12009-1-AP

    IHC staining of human tonsillitis using 12009-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 12009-1-AP

    IF Staining of mouse liver using 12009-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CAMP antibody (12009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat liver using 12009-1-AP

    IF Staining of rat liver using 12009-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CAMP antibody (12009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CAMP Polyclonal antibody

CAMP Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 12009-1-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

CAP18, Antibacterial peptide FALL-39, Antibacterial peptide FF-33, Antibacterial peptide KR-20, Antibacterial peptide KS-30

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma, mouse spleen tissue
Positive IHC detected inmouse spleen tissue, human lung cancer tissue, human spleen tissue, human tonsillitis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue, mouse liver tissue
Positive IF/ICC detected inHeLa cells, THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12009-1-AP targets CAMP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag2622

Product name: Recombinant human CAMP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-170 aa of BC055089

Sequence: MKTQRDGHSLGRWSLVLLLLGLVMPLAIIAQVLSYKEAVLRAIDGINQRSSDANLYRLLDLDPRPTMDGDPDTPKPVSFTVKETVCPRTTQQSPEDCDFKKDGLVKRCMGTVTLNQARGSFDISCDKDNKRFALLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathelicidin antimicrobial peptide
别名 CAP18, Antibacterial peptide FALL-39, Antibacterial peptide FF-33, Antibacterial peptide KR-20, Antibacterial peptide KS-30
计算分子量 170 aa, 19 kDa
观测分子量 18 kDa
GenBank蛋白编号BC055089
基因名称 CAMP
Gene ID (NCBI) 820
RRIDAB_908736
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49913
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

实验方案

Product Specific Protocols
WB protocol for CAMP antibody 12009-1-APDownload protocol
IHC protocol for CAMP antibody 12009-1-APDownload protocol
IF protocol for CAMP antibody 12009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO Mol Med

Gut microbiota-CRAMP axis shapes intestinal barrier function and immune responses in dietary gluten-induced enteropathy.

Authors - Zhengnan Ren
View Article
RatWB,IHC

Digestion

Hesperidin Improves Colonic Motility in Loeramide-Induced Constipation Rat Model via 5-Hydroxytryptamine 4R/cAMP Signaling Pathway.

Authors - Minna Wu
View Article
mouseWB

Pharm Biol

Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway

Authors - Muhua Huang
View Article
ratWB

Sci Prog

Ginsenoside Rg1 exerts anti-arrhythmic effect by inhibiting ICaL through cAMP-PKA and PI3K-AKT signaling pathways in rat ventricular myocytes after myocardial ischemia-reperfusion injury

Authors - Teng Wang
View Article
mouseIHC

Immunobiology

IL-33 synergistically promotes the proliferation of lung cancer cells in vitro by inducing antibacterial peptide LL-37 and proinflammatory cytokines in macrophages.

Authors - Yinting Jiang
View Article
mouseWB

Res Vet Sci

Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.

Authors - Lingxia Li
View Article