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验证数据展示

  • WB analysis using 15528-1-AP (same clone as 15528-1-PBS)

    WB analysis using 15528-1-AP (same clone as 15528-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15528-1-AP (CABC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15528-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15528-1-AP (same clone as 15528-1-PBS)

    IF Staining of HepG2 using 15528-1-AP (same clone as 15528-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15528-1-AP (CABC1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin. This data was developed using the same antibody clone with 15528-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 15528-1-AP (same clone as 15528-1-PBS)

    IP experiment of mouse heart using 15528-1-AP (same clone as 15528-1-PBS)

    IP result of anti-CABC1 (IP:15528-1-AP, 4ug; Detection:15528-1-AP 1:300) with mouse heart tissue lysate 3700 ug. This data was developed using the same antibody clone with 15528-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 15528-1-AP (same clone as 15528-1-PBS)

    IHC staining of human stomach using 15528-1-AP (same clone as 15528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15528-1-AP (CABC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15528-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CABC1 Polyclonal antibody, PBS Only

CABC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15528-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Chaperone activity of bc1 complex-like, Chaperone ABC1 like, Atypical kinase COQ8A, mitochondrial, ARCA2, ADCK3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15528-1-PBS targets CABC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7612

Product name: Recombinant human CABC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-647 aa of BC005171

Sequence: VRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARMKGGREVAMKIQYPGVAQSINSDVNNLMAVLNMSNMLPEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEIRNEICYNILVLCLRELFEFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAKSIEMKFLTGYEVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLVPPPEETYSLHRKMGGSFLICSKLKARFPCKAMFEEAYSNYCKRQAQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chaperone, ABC1 activity of bc1 complex homolog (S. pombe)
别名 Chaperone activity of bc1 complex-like, Chaperone ABC1 like, Atypical kinase COQ8A, mitochondrial, ARCA2, ADCK3
计算分子量 72 kDa
观测分子量 60 kDa
GenBank蛋白编号BC005171
基因名称 CABC1
Gene ID (NCBI) 56997
RRIDAB_2066403
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NI60
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CABC1 (Chaperone, ABC1 activity of bc1 complex homolog) is induced by the tumor suppressor p53 and is similar to the yeast ABC1, which is involved in an electron-transferring membrane protein complex in the respiratory chain. The CABC1 product was located in mitochondria, and may play an important role in mediating p53-inducible apoptosis. Four isoforms of CABC1 exist due to alternative splicing events.

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