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验证数据展示

  • WB analysis using 32100-1-AP

    WB analysis using 32100-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32100-1-AP (C2orf69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 32100-1-AP

    IHC staining of mouse kidney using 32100-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32100-1-AP (C2orf69 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C2orf69 Polyclonal antibody

C2orf69 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32100-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Chromosome 2 Open Reading Frame 69, COXPD53, FLJ38973, Hypothetical Protein FLJ38973, Mitochondrial protein C2orf69

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse stomach tissue, rat stomach tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32100-1-AP targets C2orf69 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 C2orf69 fusion protein Ag36779 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 2 open reading frame 69
别名 Chromosome 2 Open Reading Frame 69, COXPD53, FLJ38973, Hypothetical Protein FLJ38973, Mitochondrial protein C2orf69
计算分子量43 kDa
观测分子量50 kDa
GenBank蛋白编号BC036456
基因名称 C2orf69
Gene ID (NCBI) 205327
RRIDAB_3670190
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8N8R5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromosome 2 open reading frame 69 (C2orf69, also known as COXPD53) is located in the mitochondrion. It can affect mitochondrial membrane potential and oxidative respiration in cultured neurons and controls the levels of the glycogen branching enzyme 1 (GBE1) (PMID: 34038740; 33945503). The variant in C2orf69 probably causes developmental regression, seizures, microcephaly, autistic features, and hypertonia (PMID: 37337918). Its deficiency can disrupt the development and homeostasis of the immune and central nervous systems (PMID: 34038740).

实验方案

Product Specific Protocols
WB protocol for C2orf69 antibody 32100-1-APDownload protocol
IHC protocol for C2orf69 antibody 32100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols