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RALGAPA2 Polyclonal antibody

RALGAPA2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29843-1-AP

Print datasheet

Synonyms

250 kDa substrate of Akt, AS250, bA287B20.1, C20orf74, dJ1049G11, dJ1049G11.4, FLJ12819, KIAA1272, p220, RALGAPA2

验证数据展示

  • WB analysis using 29843-1-AP

    WB analysis using 29843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29843-1-AP (RALGAPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29843-1-AP

    IHC staining of mouse testis using 29843-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29843-1-AP (C20orf74 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HUVEC cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29843-1-AP targets RALGAPA2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen RALGAPA2 fusion protein Ag31460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 20 open reading frame 74
Synonyms 250 kDa substrate of Akt, AS250, bA287B20.1, C20orf74, dJ1049G11, dJ1049G11.4, FLJ12819, KIAA1272, p220, RALGAPA2
Calculated Molecular Weight211KD
Observed Molecular Weight230 kDa
GenBank Accession NumberNM_020343
Gene Symbol C20orf74
Gene ID (NCBI) 57186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2PPJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALGAPA2 antibody 29843-1-APDownload protocol
IHC protocol for RALGAPA2 antibody 29843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols