验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27969-1-AP targets C1orf2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | C1orf2 fusion protein Ag27428 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 1 open reading frame 2 |
| 别名 | Protein ENTREP3, Protein COTE1, FAM189B, ENTREP3, Endosomal transmembrane epsin interactor 3 |
| 计算分子量 | 669 aa, 71 kDa |
| 观测分子量 | 69-71 kDa |
| GenBank蛋白编号 | BC006493 |
| 基因名称 | C1orf2 |
| Gene ID (NCBI) | 10712 |
| RRID | AB_3086017 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P81408 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C1orf2 is also named COTE1, FAM189B and ENTREP3. C1orf2 is identified as a potential binding partner of a WW domain-containing protein which is involved in apoptosis and tumor suppression. Alternative splicing results in multiple transcript variants. COTE1 contributes to human hepatocarcinogenesis by regulating cell proliferation through the modulation of WWOX signaling, and it upregulated in hepatocellular carcinoma specimens and HCC cell lines (PMID: 23317259, PMID: 24899407).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C1orf2 antibody 27969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |