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验证数据展示

  • WB analysis using 27863-1-AP

    WB analysis using 27863-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27863-1-AP (C1orf112 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

C1orf112 Polyclonal antibody

C1orf112 Polyclonal Antibody for WB, ELISA
Cat No. 27863-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

APOLO1, FIDGETIN-like-1 interacting protein, FIGNL1 Interacting Regulator Of Recombination And Mitosis, FIGNL1-interacting regulator of recombination and mitosis, FIRRM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, K-562 cells, SH-SY5Y cells, SK-N-SH cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27863-1-AP targets C1orf112 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 C1orf112 fusion protein Ag27316 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 112
别名 APOLO1, FIDGETIN-like-1 interacting protein, FIGNL1 Interacting Regulator Of Recombination And Mitosis, FIGNL1-interacting regulator of recombination and mitosis, FIRRM
计算分子量 853 aa, 97 kDa
观测分子量98 kDa
GenBank蛋白编号BC107168
基因名称 C1orf112
Gene ID (NCBI) 55732
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSG2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FIRRM (Fanconi anemia pathway and RAD51-mediated DNA repair regulator, also known as C1orf112 or FLIP) is an evolutionarily conserved protein involved in DNA damage repair and homologous recombination. It ensures DNA cross-link (ICL) repair and genomic stability by regulating RAD51 activity. FIRRM expression is upregulated in a variety of tumors and correlates with poor prognosis. Its dysfunction leads to increased genomic instability, as evidenced by enhanced DNA damage signaling, chromosomal abnormalities, and micronucleus formation. The study of FIRRM is important for understanding the mechanisms of tumorigenesis and developing new therapeutic strategies.

实验方案

Product Specific Protocols
WB protocol for C1orf112 antibody 27863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols