验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, K-562 cells, SH-SY5Y cells, SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27863-1-AP targets C1orf112 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27316 Product name: Recombinant human C1orf112 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-530 aa of BC107168 Sequence: AIQDRILPNLSCMFALLLADRSWLLEQHTLEAFTQFAEGTNHEEIVPQCLSSEETKNKVVSFLEKTGFVDETEAAKVERVKQEKGIFWEPFANVTVEEAKRSSLQPYAKRARQEFPWEEEYRSALHTIAGALEATESLLQKGPAPAWLSMEMEALQERMDKLKRYIHTLG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 1 open reading frame 112 |
| 别名 | APOLO1, FIDGETIN-like-1 interacting protein, FIGNL1 Interacting Regulator Of Recombination And Mitosis, FIGNL1-interacting regulator of recombination and mitosis, FIRRM |
| 计算分子量 | 853 aa, 97 kDa |
| 观测分子量 | 98 kDa |
| GenBank蛋白编号 | BC107168 |
| 基因名称 | C1orf112 |
| Gene ID (NCBI) | 55732 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSG2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FIRRM (Fanconi anemia pathway and RAD51-mediated DNA repair regulator, also known as C1orf112 or FLIP) is an evolutionarily conserved protein involved in DNA damage repair and homologous recombination. It ensures DNA cross-link (ICL) repair and genomic stability by regulating RAD51 activity. FIRRM expression is upregulated in a variety of tumors and correlates with poor prognosis. Its dysfunction leads to increased genomic instability, as evidenced by enhanced DNA damage signaling, chromosomal abnormalities, and micronucleus formation. The study of FIRRM is important for understanding the mechanisms of tumorigenesis and developing new therapeutic strategies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C1orf112 antibody 27863-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
