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验证数据展示

  • IHC staining of human tonsillitis using 24553-1-AP

    IHC staining of human tonsillitis using 24553-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 24553-1-AP

    IHC staining of human tonsillitis using 24553-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of A431 using 24553-1-AP

    WB analysis of A431 using 24553-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

C15orf53 Polyclonal antibody

C15orf53 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24553-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C15orf53

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24553-1-AP targets C15orf53 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 C15orf53 fusion protein Ag18630 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 15 open reading frame 53
别名 C15orf53
计算分子量 179 aa, 20 kDa
观测分子量 ~30 kDa
GenBank蛋白编号BC119016
基因名称 C15orf53
Gene ID (NCBI) 400359
RRIDAB_2879605
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NAA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C15orf53 has been identified on chromosome 15q14. It is probably neither causative for the etiology of BD (Bipolar disorder) nor for SCZD10 (periodic catatonia). Gene expression analysis revealed that C15orf53 was expressed in a subpopulation of leukocytes, but not in human post-mortem limbic brain tissue. In our detection this antibody always detect ~30 kDa band even the amino acids is 20 kDa.

实验方案

Product Specific Protocols
WB protocol for C15orf53 antibody 24553-1-APDownload protocol
IHC protocol for C15orf53 antibody 24553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols