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验证数据展示

  • WB analysis using 26320-1-AP

    WB analysis using 26320-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26320-1-AP (C13orf3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

C13orf3 Polyclonal antibody

C13orf3 Polyclonal Antibody for WB, ELISA
Cat No. 26320-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RAMA1, SKA3, Spindle And Kinetochore Associated Complex Subunit 3, Spindle and kinetochore-associated protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26320-1-AP targets C13orf3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 C13orf3 fusion protein Ag23684 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 13 open reading frame 3
别名 RAMA1, SKA3, Spindle And Kinetochore Associated Complex Subunit 3, Spindle and kinetochore-associated protein 3
观测分子量54 kDa
GenBank蛋白编号BC048988
基因名称 C13orf3
Gene ID (NCBI) 221150
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IX90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C13orf3 (now known as Ska3) is a component of a spindle- and mitophore-associated protein complex that regulates microtubule attachment to the mitophore during mitosis. Ska3 is localized to the outer side of the mitophore during mitosis and may be essential for normal chromosome segregation. In addition, deletion of Ska3 results in a delay in mid-mitotic division and an inability to silence the spindle checkpoint or enter prophase. Accumulation of Ska3 is dependent on Sgo1 proteins, whereas mitotic accumulation of Sgo1 in prophase is not dependent on Ska3. The discovery of Ska3 further refines the function of the Ska complex, which plays an important role in mitotic progression and chromosome segregation. The discovery of Ska3 in the prophase of Sgo1 is a key factor in mitosis and chromosome segregation.

实验方案

Product Specific Protocols
WB protocol for C13orf3 antibody 26320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols