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Bleomycin hydrochloride

CAS号

67763-87-5

分子式

C55H84ClN17O21S3(Average)

主要靶点

DNA/RNA Synthesis

仅限科研使用

Cat No : CM11042

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Synonyms

DNASynthesis|DNA Synthesis|DNA synthesis|Bleomycin Hydrochloride|Bleomycinhydrochloride|RNA Synthesis|RNASynthesis|盐酸博来霉素|盐酸博莱霉素|盐酸博莱霉素

验证数据展示

  • CAS No.: 67763-87-5

    Bleomycin hydrochloride
    CAS#: 67763-87-5

产品信息

CAS号 67763-87-5
分子式 C55H84ClN17O21S3(Average)
主要靶点 DNA/RNA Synthesis
主要通路 细胞周期|DNA 损伤和修复
分子量 1451.01(Average)
纯度 ， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 store at low temperature,keep away from direct sunlight | Shipping with blue ice.
别名 DNASynthesis|DNA Synthesis|DNA synthesis|Bleomycin Hydrochloride|Bleomycinhydrochloride|RNA Synthesis|RNASynthesis|盐酸博来霉素|盐酸博莱霉素|盐酸博莱霉素

体内活性

Bleomycin hydrochloride处理（3.5-4.0 mg/kg；气管内）小鼠体重至第4天减轻，然后至第7天开始增加，直至研究结束[3]。Bleomycin hydrochloride（3.5-4.0 mg/kg；气管内）显著增加肺部羟脯氨酸水平，并增加右侧尾肺叶质量[3]。Bleomycin hydrochloride（气管内给药；5.0 mg/kg/天）在约20-30 g的八周龄雄性BALB/c小鼠中诱发肺纤维化。Bleomycin上调α-SMA和Ⅰ型胶原的表达水平[4]。Bleomycin hydrochloride（气管内；2.5 mg/kg；1.25 mg/ml，约50 μl/只小鼠）在平均体重约24.5g的八周龄雄性C57BL/6小鼠中诱导肺纤维化[5]。

体外活性

Bleomycin hydrochloride 被认为是研究最为深入的人类淋巴细胞微核诱导剂，具有不同的遗传毒性机制。Bleomycin引起的DNA损伤主要是单链和双链断裂以及单个脱氧核酮糖/嘧啶位点损伤。同时，Bleomycin也是一种真正的辐射模拟化合物，几乎完全模拟了电离辐射的遗传效应[1]。Bleomycin hydrochloride对UT-SCC-19A细胞系的IC50值为4.0±1.3 nM。UT-SCC-12A和UT-SCC-12B对Bleomycin的抗性更强，其IC50值分别为14.2±2.8 nM和13.0±1.1 nM[2]。Bleomycin hydrochloride（50、100 μM；24、48小时）可诱导RLE-6TN细胞（50 μM）和A549细胞（100 μM）发生肺纤维化[4]。

溶解度

DMSO:45 mg/mL (31 mM);H2O:80 mg/mL (55.13 mM)

参考文献

1.Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49.
2.Jaaskela-Saari HA, et al. Squamous cell cancer cell lines: sensitivity to bleomycin and suitability for animal xenograft studies. Acta Otolaryngol Suppl. 1997;529:241-4.
3.Corboz MR, et al. Therapeutic administration of inhaled INS1009, a LRX-15 prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103.
4.Kang Miao, et al. Scutellarein inhibits BLM-mediated pulmonary fibrosis by affecting fibroblast differentiation, proliferation, and apoptosis. Ther Adv Chronic Dis. 2020 Jul 30;11:2040622320940185.
5.Ling Peng, et al. Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. Cell Death Dis. 2020 Nov 13;11(11):978.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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