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验证数据展示

  • IF Staining of HepG2 using 10123-2-AP

    IF Staining of HepG2 using 10123-2-AP

    Immunofluorescent analysis of HepG2 cells, using BUB3 antibody 10123-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

BUB3 Polyclonal antibody

BUB3 Polyclonal Antibody for IF, ELISA
Cat No. 10123-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF, ELISA

别名

BUB3, BUB3L, hBUB3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BUB3 Ready-To-Use IHC Kit. BUB3 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10123-2-AP targets BUB3 in IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BUB3 fusion protein Ag0167 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 3 homolog (yeast)
别名 BUB3, BUB3L, hBUB3
计算分子量 37 kDa
GenBank蛋白编号BC005138
基因名称 BUB3
Gene ID (NCBI) 9184
RRIDAB_2066209
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43684
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BUB3 is a conserved component of the mitotic spindle assembly complex and is an essential compent of spindle-assembly checkpoint (SAC) signaling that operates during early embryogenesis. BUB3 null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. In mitosis, the SAC prevents anaphase onset until all chromosomes have been attached to the spindle microtubules and aligned correctly at the equatorial metaphase plate. BUB3 is involved in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments in mammalian oocyte meiosis.

实验方案

Product Specific Protocols
IF protocol for BUB3 antibody 10123-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols