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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 15964-1-AP (same clone as 15964-1-PBS)

    WB analysis using 15964-1-AP (same clone as 15964-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15964-1-AP (BNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

    IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

    IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15964-1-AP (same clone as 15964-1-PBS)

    IP experiment of mouse brain using 15964-1-AP (same clone as 15964-1-PBS)

    IP result of anti-BNIP1 (IP:15964-1-AP, 3ug; Detection:15964-1-AP 1:500) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

BNIP1 Polyclonal antibody, PBS Only

BNIP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15964-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15964-1-PBS targets BNIP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8737

Product name: Recombinant human BNIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-228 aa of BC010959

Sequence: MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALALFLATVLYIVKKRLFPFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2/adenovirus E1B 19kDa interacting protein 1
别名
计算分子量 228 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC010959
基因名称 BNIP1
Gene ID (NCBI) 662
RRIDAB_2066636
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12981
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.