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Species Reactivity
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验证数据展示

  • WB analysis of human colon using 14941-1-AP (same clone as 14941-1-PBS)

    WB analysis of human colon using 14941-1-AP (same clone as 14941-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14941-1-AP (same clone as 14941-1-PBS)

    IHC staining of human colon cancer using 14941-1-AP (same clone as 14941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14941-1-AP (same clone as 14941-1-PBS)

    IHC staining of human colon cancer using 14941-1-AP (same clone as 14941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skin using 14941-1-AP (same clone as 14941-1-PBS)

    IP experiment of mouse skin using 14941-1-AP (same clone as 14941-1-PBS)

    IP result of anti-BLMH (IP:14941-1-AP, 4ug; Detection:14941-1-AP 1:1000) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14941-1-AP (same clone as 14941-1-PBS)

    IF Staining of HeLa using 14941-1-AP (same clone as 14941-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14941-1-AP (BLMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

BLMH Polyclonal antibody, PBS Only

BLMH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14941-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14941-1-PBS targets BLMH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BLMH fusion protein Ag6888 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bleomycin hydrolase
别名
计算分子量 53 kDa
观测分子量 50 kDa
GenBank蛋白编号BC003616
基因名称 BLMH
Gene ID (NCBI) 642
RRIDAB_2064803
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13867
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865).