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BIK Monoclonal antibody

BIK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B1C10

Cat No : 68438-1-Ig

Print datasheet

Synonyms

Apoptosis inducer NBK, BIK, BIP1, BP4, NBK

验证数据展示

  • WB analysis using 68438-1-Ig

    WB analysis using 68438-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68438-1-Ig (BIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSW480 cells, Caco-2 cells, THP-1 cells, Raji cells, Daudi cells, Ramos cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68438-1-Ig targets BIK in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen BIK fusion protein Ag6353 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name BCL2-interacting killer (apoptosis-inducing)
Synonyms Apoptosis inducer NBK, BIK, BIP1, BP4, NBK
Calculated Molecular Weight 18 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC001599
Gene Symbol BIK
Gene ID (NCBI) 638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13323
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

实验方案

Product Specific Protocols
WB protocol for BIK antibody 68438-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols