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验证数据展示

  • WB analysis using 25699-1-AP (same clone as 25699-1-PBS)

    WB analysis using 25699-1-AP (same clone as 25699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25699-1-AP (BDNF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 25699-1-AP (same clone as 25699-1-PBS)

    IHC staining of mouse cerebellum using 25699-1-AP (same clone as 25699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 25699-1-AP (same clone as 25699-1-PBS)

    IHC staining of mouse cerebellum using 25699-1-AP (same clone as 25699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 25699-1-AP (same clone as 25699-1-PBS)

    IF Staining of PC-12 using 25699-1-AP (same clone as 25699-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (25699-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BDNF Polyclonal antibody, PBS Only

BDNF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25699-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25699-1-PBS targets BDNF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22489

Product name: Recombinant human BDNF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-179 aa of BC029795

Sequence: SDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brain-derived neurotrophic factor
别名 Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form
计算分子量 247 aa, 28 kDa
观测分子量30 kDa
GenBank蛋白编号BC029795
基因名称 BDNF
Gene ID (NCBI) 627
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23560
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

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