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验证数据展示

  • WB analysis of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    WB analysis of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 19064-1-AP (same clone as 19064-1-PBS)

    IHC staining of human testis using 19064-1-AP (same clone as 19064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 19064-1-AP (same clone as 19064-1-PBS)

    IHC staining of human testis using 19064-1-AP (same clone as 19064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    WB analysis of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    IP experiment of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    IP result of anti-BAG1 (IP:19064-1-AP, 3ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 3440ug. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    IF Staining of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAG1 antibody (19064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BAG1 Polyclonal antibody, PBS Only

BAG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19064-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

19064-1-PBS targets BAG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 BAG1 fusion protein Ag13555 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2-associated athanogene
别名
计算分子量 39 kDa
观测分子量 50-55 kDa, 30-33 kDa
GenBank蛋白编号BC014774
基因名称 BAG1
Gene ID (NCBI) 573
RRIDAB_10640574
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99933
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.