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验证数据展示

  • WB analysis of HEK-293 using 14018-1-AP (same clone as 14018-1-PBS)

    WB analysis of HEK-293 using 14018-1-AP (same clone as 14018-1-PBS)

    WB result of BACH1 antibody (14018-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • WB analysis using 14018-1-AP (same clone as 14018-1-PBS)

    WB analysis using 14018-1-AP (same clone as 14018-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 14018-1-AP (same clone as 14018-1-PBS)

    IHC staining of human cervical cancer using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BACH1 Polyclonal antibody, PBS Only

BACH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14018-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14018-1-PBS targets BACH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 BACH1 fusion protein Ag5128 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BTB and CNC homology 1, basic leucine zipper transcription factor 1
别名
计算分子量 82 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC063307
基因名称 BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
RRIDAB_2274498
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14867
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle 

regulation in homeostasis and in cancer in disease.


What is the molecular weight of BACH1?

Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor 

family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs 

anomalously at 100-110 kDa in SDS-PAGE.


What is the cellular localization of BACH1?

As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a 

heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the 

Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by 

heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to 

microtubules (PMID: 15809329).


What is the function of BACH1?

BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme 

oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved 

in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor 

(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a 

role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6 

(CDK6) and BCL2-like 11 (BCL2L11).


What is the role of BACH1 in disease?

BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function 

of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis, 

influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787

892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).