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B3GALNT2 Polyclonal antibody

B3GALNT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17142-1-AP

Print datasheet

Synonyms

B3GalNAc T2, B3GALNT2, Beta 1,3 GalNAc T2

验证数据展示

  • WB analysis of HEK-293 using 17142-1-AP

    WB analysis of HEK-293 using 17142-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17142-1-AP (B3GALNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 17142-1-AP

    IHC staining of human heart using 17142-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17142-1-AP (B3GALNT2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17142-1-AP targets B3GALNT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen B3GALNT2 fusion protein Ag10907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-1,3-N-acetylgalactosaminyltransferase 2
Synonyms B3GalNAc T2, B3GALNT2, Beta 1,3 GalNAc T2
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC029564
Gene Symbol B3GALNT2
Gene ID (NCBI) 148789
RRIDAB_2063040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCR0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B3GALNT2 antibody 17142-1-APDownload protocol
IHC protocol for B3GALNT2 antibody 17142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

J Hematol Oncol

Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.

Authors - Tianxiao Yang
View Article