AuthentiKine™ Human beta-NGF ELISA Kit
Validation Data Gallery
Product Information
KE00174 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The beta-NGF ELISA kit is to be used to detect and quantify protein levels of endogenous beta -NGF. The assay recognizes human beta-NGF. An antibody specific for beta -NGF has been pre-coated onto the microwells. The beta-NGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for beta-NGF is added to detect the captured beta-NGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human beta-NGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4803 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 73%-119% |
| Cell culture supernatants | 119% | 100%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 69.6 | 1.6 | 2.3 |
| 2 | 20 | 140.4 | 1.8 | 1.3 |
| 3 | 20 | 242.1 | 4.9 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 72.0 | 2.6 | 3.6 |
| 2 | 24 | 140.8 | 3.4 | 2.4 |
| 3 | 24 | 242.9 | 6.9 | 2.9 |
Background Information
NGF is a member of the neurotrophin family that regulates cell proliferation and differentiation within specific neural tissues, under both physiological and pathological condition. As NGF plays a key role in the proliferation, differentiation, and regulation of neural stem cells, several studies have indicated the clinical potential of NGF treatment in the regeneration of nerve cells following injury. NGF is initially in a 7S, 130-kDa complex of 3 proteins: alpha-NGF, beta-NGF, and gamma-NGF, while the beta subunit, exhibits all the biological activities ascribed to NGF. Beta-NGF stimulates the growth of sympathetic and some sensory neurites.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF, β-NGF |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Commun Biol Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor | ||
Neuropsychiatr Dis Treat Hyperbaric Oxygen Therapy Upregulates Neurotrophic Factors to Ameliorate Post-Stroke Depression: A Randomized Sham-Controlled Trial. | ||
J Control Release M2 macrophage-derived exosomes deliver NGF-modified mRNA to promote comprehensive corneal repair. |
