AuthentiKine™ Human IL-27 ELISA Kit
Cat no : KE00182
Synonyms
IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28
Validation Data Gallery
Product Information
KE00182 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous IL27. The assay recognizes human IL27. An antibody specific for IL27 has been pre-coated onto the microwells. The IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL27 is added to detect the captured IL27 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | AuthentiKine™ Human IL-27 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 246778 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 80%-114% |
| Cell culture supernatants | 90% | 74%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,555.9 | 61.3 | 2.4 |
| 2 | 20 | 383.8 | 9.6 | 2.5 |
| 3 | 20 | 72.7 | 6.0 | 8.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,667.3 | 79.7 | 3.0 |
| 2 | 24 | 411.7 | 10.2 | 2.5 |
| 3 | 24 | 94.9 | 7.5 | 7.9 |
Background Information
IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Heliyon Conditional reprogrammed human limbal epithelial cell model for anti-SARS-CoV-2 drug screening |
