AuthentiKine™ Human IL-27 ELISA Kit0 Publications

Catalog number: KE00182

规格

单价

96T

¥3200

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Overview


Product name:
AuthentiKine™ Human IL-27 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma

Assay type:
Sandwich

Sensitivity:
0.1 pg/mL

Range:
78.1-5000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Human plasma 99% 80%-114%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2555.9 61.3 2.4
2 20 383.8 9.6 2.5
3 20 72.7 6.0 8.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2667.3 79.7 3.0
2 24 411.7 10.2 2.5
3 24 94.9 7.5 7.9

Product overview:
KE00182 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous IL27. The assay recognizes human IL27. An antibody specific for IL27 has been pre-coated onto the microwells. The IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL27 is added to detect the captured IL27 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .

Properties


Storage Instructions:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:
IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28
Background

IL27, a member of IL12/IL23 heterodimeric family of cytokines, has pleiotropic properties that can enhance or limit immune responses. IL27 is a heterodimeric cytokine composed of two subunits: IL27a and IL27b. IL27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL10 producing type 1 regulatory T cells. IL27 is a potentially promising cytokine for therapeutic approaches on various human diseases.


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