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验证数据展示

  • WB analysis using 32628-1-AP

    WB analysis using 32628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32628-1-AP (Ace2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 32628-1-AP

    IHC staining of mouse heart using 32628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 32628-1-AP (Ace2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Ace2 Polyclonal antibody

Ace2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32628-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

ACE-related carboxypeptidase, Angiotensin-converting enzyme 2, EC:3.4.17.-, EC:3.4.17.23, Processed angiotensin-converting enzyme 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse small intestine tissue, rat small intestine tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32628-1-AP targets Ace2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Ace2 fusion protein Eg2834 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
别名 ACE-related carboxypeptidase, Angiotensin-converting enzyme 2, EC:3.4.17.-, EC:3.4.17.23, Processed angiotensin-converting enzyme 2
计算分子量92 kDa
观测分子量110 kDa, 120 kDa
GenBank蛋白编号NM_001130513.1
基因名称 Ace2
Gene ID (NCBI) 70008
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8R0I0-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACE2 (Angiotensin-Converting Enzyme 2) is a key regulator of the renin-angiotensin system (RAS) and plays a crucial role in maintaining cardiovascular homeostasis. It is also the functional receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19.

实验方案

Product Specific Protocols
WB protocol for Ace2 antibody 32628-1-APDownload protocol
IHC protocol for Ace2 antibody 32628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols