AbFlex Histone H3, C-terminal antibody (rAb, Rabbit)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, WB

Cat No : 91299,91300 91299

Synonyms

AbFlex, recombinant antibody, Histone H3, H3 c-terminal, antibody,



产品信息

Tested Applications ChIP, WB

Applications Validated by Active Motif: ChIP: 4 - 5 ug per ChIP WB*: 0.01 – 0.5 ug/ml *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen This antibody was raised against a peptide at the C-terminus of human Histone H3.
Full Name AbFlex Histone H3, C-terminal antibody (rAb, Rabbit)
Synonyms AbFlex, recombinant antibody, Histone H3, H3 c-terminal, antibody,
Molecular weight 17 kDa
GenBank accession numberNP_003522
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3, C-term was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in CHO cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4).