AbFlex Histone H2A.XS139ph antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Wide Range Predicted

Applications

WB

Cat No : 91395,91396 91395

Synonyms

Histone H2AX, H2A histone family member X, H2AX P-S139, antibody, antibodies,AbFlex, H2AXS139Ph, 39117



产品信息

Tested Applications WB

Validated Applications: WB:0.5 – 2 ug/ml *Note: cell lysates prepared by acid extraction is recommended for western blot. Please refer to the method outlines in Active Motif Histone Extraction Kit Manual, Cat. No. 40028, section B, for the protocol we use to prepare acid extracts from cells.

Tested Reactivity Wide Range Predicted
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against a phospho-peptide corresponding to phospho-Histone H2AX (Ser139).
Full Name AbFlex Histone H2A.XS139ph antibody (rAb)
Synonyms Histone H2AX, H2A histone family member X, H2AX P-S139, antibody, antibodies,AbFlex, H2AXS139Ph, 39117
Molecular weight 15 kDa
GenBank accession numberNP_002096
Purification Method Protein A Chromatography
Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation