AbFlex Cas9 antibody (rAb)
Host / Isotype
Mouse / IgG2a
ChIP, ChIP-Seq, ELISA, WB
Cat No : 91123,91124 91123
ChIP, ChIP-Seq, ELISA, WB
Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP Bead-based ELISA: 9 - 75 ng/ml WB: 2 ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61577.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody recognizes Cas9 and dCas9.|
|Full Name||AbFlex Cas9 antibody (rAb)|
|Synonyms||Cas9, Streptococcus pyogenes, CRISPR, clustered regularly interspaced short palindromic repeat, gene-editing, guide RNA, nuclease, endonuclease, targeting, DNA, double-strand break, RNA, genome editing, wb, ip, if, western blotting, immunoprecipitation, immunoflourescence, antibody, antibodies, sample, recombinant, rAb, rec Ab, AbFlex|
|Molecular weight||160 kDa|
|GenBank accession number||WP_014407541||RRID||AB_2793783||Purification Method||Ni-NTA|
|Buffer||Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.|
|Storage||Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.|
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Cas9 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.