验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, rabbit heart tissue, LNCaP cells, mouse heart tissue, mouse liver tissue, rat liver tissue, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
68128-1-Ig targets ATP5J2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, Mouse, Rat, Rabbit |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8811 Product name: Recombinant human ATP5J2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-94 aa of BC003678 Sequence: MASVGECPAPVPVKDKKLLEVKLLELPSWILMRDFSPSGIFGAFQRGYYRYYNKYINVKKGSISGITMVLACYVLFSYSFSYKHLKHERLRKYH 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2 |
| 别名 | ATP5J2, ATP5JL |
| 计算分子量 | 5-11 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC003678 |
| 基因名称 | ATP5J2 |
| Gene ID (NCBI) | 9551 |
| RRID | AB_2923655 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P56134 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP5J2 antibody 68128-1-Ig | Download protocol |
| WB protocol for ATP5J2 antibody 68128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration | ||
J Transl Med Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC | ||
