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ATP5J2 Monoclonal antibody

ATP5J2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B3C3

Cat No : 68128-1-Ig

Print datasheet

Synonyms

ATP5J2, ATP5JL

验证数据展示

  • WB analysis using 68128-1-Ig

    WB analysis using 68128-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 68128-1-Ig

    IF Staining of MCF-7 using 68128-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat heart tissue, rabbit heart tissue, LNCaP cells, mouse heart tissue, mouse liver tissue, rat liver tissue, HeLa cells, HepG2 cells
Positive IF detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68128-1-Ig targets ATP5J2 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Rabbit
Immunogen ATP5J2 fusion protein Ag8811 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
Synonyms ATP5J2, ATP5JL
Calculated Molecular Weight 5-11 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC003678
Gene Symbol ATP5J2
Gene ID (NCBI) 9551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP56134
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP5J2 antibody 68128-1-IgDownload protocol
IF protocol for ATP5J2 antibody 68128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols