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验证数据展示

  • WB analysis of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

    WB analysis of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • WB analysis using 15192-1-AP (same clone as 15192-1-PBS)

    WB analysis using 15192-1-AP (same clone as 15192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 15192-1-AP (same clone as 15192-1-PBS)

    IF Staining of HEK-293 using 15192-1-AP (same clone as 15192-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 15192-1-AP (ATP1B1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

    IP experiment of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

    IP result of anti-ATP1B1 (IP:15192-1-AP, 4ug; Detection:15192-1-AP 1:2000) with mouse brain tissue lysate 1600 ug. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

ATP1B1 Polyclonal antibody, PBS Only

ATP1B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15192-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15192-1-PBS targets ATP1B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7279

Product name: Recombinant human ATP1B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 59-303 aa of BC000006

Sequence: LTISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 1 polypeptide
别名 Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase
计算分子量 35 kDa
观测分子量 45-52 kDa
GenBank蛋白编号BC000006
基因名称 ATP1B1
Gene ID (NCBI) 481
RRIDAB_2290040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05026
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

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