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验证数据展示

  • WB analysis using 29770-1-AP

    WB analysis using 29770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29770-1-AP (ATF7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 29770-1-AP

    IHC staining of mouse lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of mouse lung using 29770-1-AP

    IHC staining of mouse lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of human lung using 29770-1-AP

    IHC staining of human lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of human lung using 29770-1-AP

    IHC staining of human lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of human urothelial carcinoma using 29770-1-AP

    IHC staining of human urothelial carcinoma using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of rat heart using 29770-1-AP

    IHC staining of rat heart using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of rat lung using 29770-1-AP

    IHC staining of rat lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included

ATF7 Polyclonal antibody

ATF7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29770-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

ATFA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung tissue, mouse lung tissue, human urothelial carcinoma tissue, mouse heart tissue, rat heart tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATF7 Ready-To-Use IHC Kit. ATF7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29770-1-AP targets ATF7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag30882

Product name: Recombinant human ATF7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 78-167 aa of NM_001366555

Sequence: FKKAADEDEKKARSRTVAKKLVAAAGPLDMSLPSTPDIKIKEEEPVEVDSSPPDSPASSPCSPPLKEKEVTPKPVLISTPTPTIVRPGSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activating transcription factor 7
别名 ATFA
计算分子量 53 kDa
观测分子量52-60 kDa
GenBank蛋白编号NM_001366555
基因名称 ATF7
Gene ID (NCBI) 11016
RRIDAB_3086157
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ATF7 antibody 29770-1-APDownload protocol
WB protocol for ATF7 antibody 29770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Acta Pharmacol Sin

CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Authors - Yi-Hong Wen
View Article
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