Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.