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验证数据展示

  • IHC staining of mouse kidney using 25693-1-AP

    IHC staining of mouse kidney using 25693-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 25693-1-AP

    IHC staining of mouse kidney using 25693-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 25693-1-AP

    IF Staining of A431 using 25693-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATAD4 / PRR15L antibody (25693-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ATAD4 / PRR15L Polyclonal antibody

ATAD4 / PRR15L Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 25693-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

ATAD4, Protein ATAD4, PRR15L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25693-1-AP targets ATAD4 / PRR15L in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ATAD4 / PRR15L fusion protein Ag22500 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase family, AAA domain containing 4
别名 ATAD4, Protein ATAD4, PRR15L
计算分子量 103 aa, 12 kDa
GenBank蛋白编号BC002865
基因名称 ATAD4
Gene ID (NCBI) 79170
RRIDAB_3085816
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BU68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATAD4 (ATPase family AAA domain-containing protein 4), also known as PRR15L (Proline Rich 15 Like), and its function has not yet been discovered.

实验方案

Product Specific Protocols
IHC protocol for ATAD4 / PRR15L antibody 25693-1-APDownload protocol
IF protocol for ATAD4 / PRR15L antibody 25693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols