• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATAD2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

Cat No : 61369,61370 61369

Synonyms

ATAD2, ATPase family, AAA domain containing 2, ANCCA, PRO2000, ATPase family AAA domain-containing protein 2, AAA nuclear coregulator cancer-associated protein, western blotting, wb, immunoprecipitation, ip, antibody, polyclonal, pAb, sample

验证数据展示

  • ATAD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of ATAD2 antibody was used to immunoprecipitate ATAD2 from 250 ug of Jurkat nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ATAD2 antibody at a dilution of 1:1,000.

    ATAD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of ATAD2 antibody was used to immunoprecipitate ATAD2 from 250 ug of Jurkat nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ATAD2 antibody at a dilution of 1:1,000.

  • ATAD2 antibody (pAb) tested by Western blot. ATAD2 detection by Western blot. The analysis was performed using 40 ug Jurkat nuclear cell extract and CENP-B at a 1:1,000 dilution.

    ATAD2 antibody (pAb) tested by Western blot. ATAD2 detection by Western blot. The analysis was performed using 40 ug Jurkat nuclear cell extract and CENP-B at a 1:1,000 dilution.

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 5 - 10 ul per IP WB*: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of human ATAD2.
Full Name ATAD2 antibody (pAb)
Synonyms ATAD2, ATPase family, AAA domain containing 2, ANCCA, PRO2000, ATPase family AAA domain-containing protein 2, AAA nuclear coregulator cancer-associated protein, western blotting, wb, immunoprecipitation, ip, antibody, polyclonal, pAb, sample
Molecular weight 160 kDa
GenBank accession numberNP_054828
RRIDAB_2793609
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

ATAD2 (ATPase family AAA domain-containing protein 2) may be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.