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验证数据展示

  • WB analysis of mouse testis using 11354-1-AP

    WB analysis of mouse testis using 11354-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11354-1-AP (ART5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

ART5 Polyclonal antibody

ART5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11354-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11354-1-AP targets ART5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ART5 fusion protein Ag1786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosyltransferase 5
别名 ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5
计算分子量 292 aa, 32 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014577
基因名称 ART5
Gene ID (NCBI) 116969
RRIDAB_10734329
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96L15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ART5 (Ecto-ADP-ribosyltransferase 5) belongs to a class of enzymes that catalyze the transfer of an ADP-ribose group from NAD to arginine residues on target proteins like G proteins. ART5 is a secreted protein. This antibody detects the 43 kDa ART5 protein in mouse liver and testis.

实验方案

Product Specific Protocols
WB protocol for ART5 antibody 11354-1-APDownload protocol
IHC protocol for ART5 antibody 11354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols