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验证数据展示

  • WB analysis of mouse testis using 11354-1-AP

    WB analysis of mouse testis using 11354-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11354-1-AP (ART5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

ART5 Polyclonal antibody

ART5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11354-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11354-1-AP targets ART5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1786

Product name: Recombinant human ART5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-292 aa of BC014577

Sequence: MALAALMIALGSLGLHTWQAQAVPTILPLGLAPDTFDDTYVGCVEEMEEKAAPLLKEEMAHHALLRESWEAAQETWEDKRRGLTLPPGFKAQNGIAIMVYTNSSNTLYWELNQAVRTGGGSRELYMRHFPFKALHFYLIRALQLLRGSGGCSRGPGEVVFRGVGSLRFEPKRLGDSVRLGQFASSSLDKAVAHRFGNATLFSLTTCFGAPIQAFSVFPKEREVLIPPHEVFLVTRFSQDGAQSLVTLWSYNQTCSHFNCAYLGGEKRRGCVSAPGALGTGDLHMTKRHLQQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosyltransferase 5
别名 ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5
计算分子量 292 aa, 32 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014577
基因名称 ART5
Gene ID (NCBI) 116969
RRIDAB_10734329
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96L15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ART5 (Ecto-ADP-ribosyltransferase 5) belongs to a class of enzymes that catalyze the transfer of an ADP-ribose group from NAD to arginine residues on target proteins like G proteins. ART5 is a secreted protein. This antibody detects the 43 kDa ART5 protein in mouse liver and testis.

实验方案

Product Specific Protocols
IHC protocol for ART5 antibody 11354-1-APDownload protocol
WB protocol for ART5 antibody 11354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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