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验证数据展示

  • WB analysis using 21182-1-AP

    WB analysis using 21182-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21182-1-AP (ARHGEF39 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

ARHGEF39 Polyclonal antibody

ARHGEF39 Polyclonal Antibody for WB, ELISA
Cat No. 21182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C9orf100, Vav like protein C9orf100, RP11 331F9.7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, SMMC-7721

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21182-1-AP targets ARHGEF39 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15473

Product name: Recombinant human C9orf100 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-335 aa of BC033666

Sequence: MELSCPGSRCPVQEQRARWERKRACTARELLETERRYQEQLGLVATYFLGILKAKGTLRPPERQALFGSWELIYGASQELLPYLEGGCWGQGLEGFCRHLELYNQFAANSERSQTTLQEQLKKNKGFRRFVRLQEGRPEFGGLQLQDLLPLPLQRLQQYENLVVALAENTGPNSPDHQQLTRAARLISETAQRVHTIGQKQKNDQHLRRVQALLSGRQAKGLTSGRWFLRQGWLLVVPPHGEPRPRMFFLFTDVLLMAKPRPPLHLLRSGTFACKALYPMAQCHLSRVFGHSGGPCGGLLSLSFPHEKLLLMSTDQEELSRWYHSLTWAISSQKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 100
别名 C9orf100, Vav like protein C9orf100, RP11 331F9.7
计算分子量 335 aa, 38 kDa
观测分子量34 kDa
GenBank蛋白编号BC033666
基因名称 C9orf100
Gene ID (NCBI) 84904
RRIDAB_3669361
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4T4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rho guanine nucleotide exchange factor 39 (ARHGEF39), also called C9orf100, is part of the family of Rho guanine nucleotide exchange factors (RhoGEFs) that activate small Rho GTPases to regulate a wide variety of cellular processes. ARHGEF39 was previously implicated in developmental language disorder (DLD) via a functional polymorphism that can disrupt post-transcriptional regulation by microRNAs.

实验方案

Product Specific Protocols
WB protocol for ARHGEF39 antibody 21182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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