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验证数据展示

  • IP experiment of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    IP experiment of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    IP result of anti-ARFGAP3 (IP:15293-1-AP, 4ug; Detection:15293-1-AP 1:2000) with HepG2 cells lysate 3000 ug. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    WB analysis of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFGAP3 antibody included
  • IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFGAP3 antibody included
  • IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of A431 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARFGAP3 Polyclonal antibody, PBS Only

ARFGAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15293-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ARFGAP1, ARF GAP 3, ADP-ribosylation factor GTPase-activating protein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ARFGAP3 Ready-To-Use IHC Kit. ARFGAP3 primary antibody included.

产品信息

15293-1-PBS targets ARFGAP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7536

Product name: Recombinant human ARFGAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC005122

Sequence: MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDSNWSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSLASQATRKHGTDLWLDSCVVPPLSPPPKEEDFFASHVSPEVSDTAWASAIAEPSSLTSRPVETTLENNEGGQEQGPSVEGLNVPTKATLEVSSIIKKKPNQAKKGLGAKKGSLGAQKLANTCFNEIEKQAQAADKMKEQEDLAKVVSKEESIVSSLRLAYKDLEIQMKKDEKMNISGKKNVDSDRLGMGFGNCRSVISHSVTSDMQTIEQESPIMAKPRKKYNDDSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor GTPase activating protein 3
别名 ARFGAP1, ARF GAP 3, ADP-ribosylation factor GTPase-activating protein 3
计算分子量 57 kDa
观测分子量 57-66 kDa
GenBank蛋白编号BC005122
基因名称 ARFGAP3
Gene ID (NCBI) 26286
RRIDAB_10804035
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP61
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARFGAP3, also named as ADP-ribosylation factor GTPase-activating protein 3, is a 516 amino acid protein, which is Widely expressed with Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate). ARFGAP3 is a GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. The calculated molecular weight of ARFGAP3 is 57 kDa, but modified ARFGAP3 is about 57-66 kDa.

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