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验证数据展示

  • WB analysis of mouse brain using 10800-1-AP (same clone as 10800-1-PBS)

    WB analysis of mouse brain using 10800-1-AP (same clone as 10800-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 10800-1-AP (same clone as 10800-1-PBS)

    IHC staining of human kidney using 10800-1-AP (same clone as 10800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARF3 antibody included
  • IHC staining of human kidney using 10800-1-AP (same clone as 10800-1-PBS)

    IHC staining of human kidney using 10800-1-AP (same clone as 10800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARF3 antibody included
  • IF Staining of HepG2 using 10800-1-AP (same clone as 10800-1-PBS)

    IF Staining of HepG2 using 10800-1-AP (same clone as 10800-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARF3 antibody (10800-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 10800-1-PBS in a different storage buffer formulation.

ARF3 Polyclonal antibody, PBS Only

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10800-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ADP-ribosylation factor 3, ADP ribosylation factor 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARF3 Ready-To-Use IHC Kit. ARF3 primary antibody included.

产品信息

10800-1-PBS targets ARF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ARF3 fusion protein Ag1174 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor 3
别名 ADP-ribosylation factor 3, ADP ribosylation factor 3
计算分子量 21 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC017565
基因名称 ARF3
Gene ID (NCBI) 377
RRIDAB_2243128
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61204
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.