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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 27874-1-AP (same clone as 27874-1-PBS)

    WB analysis using 27874-1-AP (same clone as 27874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27874-1-AP (APBB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 27874-1-AP (same clone as 27874-1-PBS)

    IP experiment of mouse brain using 27874-1-AP (same clone as 27874-1-PBS)

    IP result of anti-APBB1 (IP:27874-1-AP, 4ug; Detection:27874-1-AP 1:3000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

APBB1 Polyclonal antibody, PBS Only

APBB1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 27874-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy APBB1 Ready-To-Use IHC Kit. APBB1 primary antibody included.

产品信息

27874-1-PBS targets APBB1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 APBB1 fusion protein Ag27434 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
别名
计算分子量 708 aa, 77 kDa
观测分子量 65 kDa, 97 kDa
GenBank蛋白编号BC010854
基因名称 APBB1
Gene ID (NCBI) 322
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00213
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein FE65 (APBB1) specifically binds to the intracellular tail of the type I transmembrane protein, β-amyloid precursor protein (APP). FE65 is a multimodular adaptor protein, consisting of three major protein-protein interaction domains, a WW domain and two phosphotyrosine interaction domains (PID). The interaction between FE65 and APP mainly takes place between the C-terminal PID (PID2) and the APP intracellular domain (AICD) (PMID: 15647266). There is novel evidence that the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis. The cleavage region locates immediately after an acidic residue cluster but before the three major protein-protein binding domains (PMID: 15647266). FE65 is predominantly expressed in central nervous system neurons, and its expression is regulated during development and aging, with high levels corresponding to the timing of neural tissue formation and high neuronal activity (PMID: 7867517, PMID: 10561691, PMID: 14689444). p65FE65 (65kDa) is endoproteolytically cleaved from p97FE65 (97 kDa), the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis (PMID: 15647266).