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验证数据展示

  • WB analysis using 16313-1-AP (same clone as 16313-1-PBS)

    WB analysis using 16313-1-AP (same clone as 16313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16313-1-AP (AP4B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16313-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16313-1-AP (same clone as 16313-1-PBS)

    IP experiment of HeLa using 16313-1-AP (same clone as 16313-1-PBS)

    IP result of anti-AP4B1 (IP:16313-1-AP, 4ug; Detection:16313-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16313-1-PBS in a different storage buffer formulation.

AP4B1 Polyclonal antibody, PBS Only

AP4B1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16313-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16313-1-PBS targets AP4B1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9348

Product name: Recombinant human AP4B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC014146

Sequence: MPYLGSEDVVKELKKALCNPHIQADRLRYRNVIQRVIRYMTQGLDMSGVFMEMVKASATVDIVQKKLVYLYMCTYAPLKPDLALLAINTLCKDCSDPNPMVRGLALRSMCSLRMPGVQEYIQQPILNGLRDKASYVRRVAVLGCAKMHNLHGDSEVDGALVNELYSLLRDQDPIVVVNCLRSLEEILKQEGGVVINKPIAHHLLNRMSKLDQWGQAEVLNFLLRYQPRSEEELFDILNLLDSFLKSSSPGVVMGATKLFLILAKMFPHVQTDVLVRVKGPLLAACSSESRELCFVALCHVRQILHSLPGHFSSHYKKFFCSYSEPHYIKLQKVEVLCELVNDENVQQVLEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 4, beta 1 subunit
别名 Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1
计算分子量 739 aa, 83 kDa
观测分子量75-83 kDa
GenBank蛋白编号BC014146
基因名称 AP4B1
Gene ID (NCBI) 10717
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6B7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.

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