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验证数据展示

  • WB analysis of Jurkat using 21100-1-AP

    WB analysis of Jurkat using 21100-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21100-1-AP (ANKRD13D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 21100-1-AP

    IHC staining of human small intestine using 21100-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21100-1-AP (ANKRD13D Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 21100-1-AP

    IHC staining of human breast cancer using 21100-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21100-1-AP (ANKRD13D Antibody) at dilution of 1:50 (under 40x lens).

ANKRD13D Polyclonal antibody

ANKRD13D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21100-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ANKRD13D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HT-1080 cells
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21100-1-AP targets ANKRD13D in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15369

Product name: Recombinant human ANKRD13D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 284-367 aa of BC110419

Sequence: LSDQDKSRSKAGKTPFQSFLGMAQQHSSHTGAPVQQAASPTNPTAISPEEYFDPNFSLESRNIGRPIEMSSKVQRFKATLWLSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 13 family, member D
别名 ANKRD13D
计算分子量 605 aa, 68 kDa
观测分子量 50 kDa
GenBank蛋白编号BC110419
基因名称 ANKRD13D
Gene ID (NCBI) 338692
RRIDAB_2878813
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZTN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD13D, also named as Ankyrin repeat domain-containing protein 13D, is a 518 amino acid protein, which contains 4 UIM (ubiquitin-interacting motif) repeats. ANKRD13D localizes in the cell membrane and late endosome. ANKRD13D as a ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. The calculated molecular weight of ANKRD13D is 58kDa, but we decteted a 50kDa protein via western blot. experiment.

实验方案

Product Specific Protocols
WB protocol for ANKRD13D antibody 21100-1-APDownload protocol
IHC protocol for ANKRD13D antibody 21100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols