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验证数据展示

  • WB analysis of mouse small intestine using 25737-1-AP

    WB analysis of mouse small intestine using 25737-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25737-1-AP (ANKRD13B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25737-1-AP

    IHC staining of human testis using 25737-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25737-1-AP (ANKRD13B Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 25737-1-AP

    IHC staining of human testis using 25737-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25737-1-AP (ANKRD13B Antibody) at dilution of 1:50 (under 40x lens).

ANKRD13B Polyclonal antibody

ANKRD13B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25737-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ANKRD13B, ankyrin repeat domain 13B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse small intestine tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25737-1-AP targets ANKRD13B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ANKRD13B fusion protein Ag22732 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 13B
别名 ANKRD13B, ankyrin repeat domain 13B
计算分子量 626 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC032554
基因名称 ANKRD13B
Gene ID (NCBI) 124930
RRIDAB_2880217
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANKRD13B (ankyrin repeat domain 13B) is an ubiquitin-binding protein belongs to the Ankrd 13 family. It specifically recognizes and binds 'Lys-63'-linked ubiquitin, and positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane (PMID: 22298428). This antibody specifically recognises the 70 kDa protein.

实验方案

Product Specific Protocols
WB protocol for ANKRD13B antibody 25737-1-APDownload protocol
IHC protocol for ANKRD13B antibody 25737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols