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Species Reactivity
0
Species Reactivity
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Conjugate
0
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0
Conjugate
0

验证数据展示

  • WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

    WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • Featured Product
  • KD/KO验证

ALKBH3 Polyclonal antibody, PBS Only

ALKBH3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12292-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.

产品信息

12292-1-PBS targets ALKBH3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2982

Product name: Recombinant human ALKBH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 148-286 aa of BC015155

Sequence: MEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alkB, alkylation repair homolog 3 (E. coli)
别名
计算分子量 286 aa, 33 kDa
观测分子量 22-25 kDa, 33kDa
GenBank蛋白编号BC015155
基因名称 ALKBH3
Gene ID (NCBI) 221120
RRIDAB_11125161
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96Q83
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.