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验证数据展示

  • WB analysis using 29218-1-AP (same clone as 29218-1-PBS)

    WB analysis using 29218-1-AP (same clone as 29218-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29218-1-AP (ALG9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29218-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 29218-1-AP (same clone as 29218-1-PBS)

    IHC staining of human stomach cancer using 29218-1-AP (same clone as 29218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29218-1-AP (ALG9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29218-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 29218-1-AP (same clone as 29218-1-PBS)

    IHC staining of human stomach cancer using 29218-1-AP (same clone as 29218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29218-1-AP (ALG9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29218-1-PBS in a different storage buffer formulation.

ALG9 Polyclonal antibody, PBS Only

ALG9 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29218-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EC:2.4.1.259, Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, DIBD1, Asparagine-linked glycosylation protein 9 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29218-1-PBS targets ALG9 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30489

Product name: Recombinant human ALG9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 500-582 aa of BC009255

Sequence: EFRGQLPKPFAEGPLATRIVPTDMNDQNLEEPSRYIDISKCHYLVDLDTMRETPREPKYSSNKEEWISLAYRPFLDASRSSKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae)
别名 EC:2.4.1.259, Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase, DIBD1, Asparagine-linked glycosylation protein 9 homolog
计算分子量 70 kDa
观测分子量70 kDa
GenBank蛋白编号BC009255
基因名称 ALG9
Gene ID (NCBI) 79796
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6U8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALG9 encodes an endoplasmic reticulum enzyme that builds N-glycans, the third ER protein-encoding polycystic disease gene after GANAB and DNAJB11. Autosomal recessive loss of ALG9 results in a severe congenital disorder of glycosylation (CDG) with a multiorgan phenotype that includes kidney cysts (PMID: 31395617). Western blot analysis detected ALG9 at an apparent molecular mass of 70 kDa.

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