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P5CS Polyclonal antibody

P5CS Polyclonal Antibody for WB, IP, ELISA
Cat No. 17719-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (3)

ALDH18A1, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, mouse brain tissue, HepG2 cells, MCF-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17719-1-AP targets P5CS in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 P5CS fusion protein Ag12100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 18 family, member A1
别名 ALDH18A1, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase
计算分子量 795 aa, 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC106054
基因名称 P5CS
Gene ID (NCBI) 5832
RRIDAB_2223896
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54886
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for P5CS antibody 17719-1-APDownload protocol
IP protocol for P5CS antibody 17719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

Nature

Cellular ATP demand creates metabolically distinct subpopulations of mitochondria

Authors - Keun Woo Ryu
  • KO Validated
humanWB

Sci Adv

Loss of attachment promotes proline accumulation and excretion in cancer cells

Authors - Steven E Pilley
humanWB

J Exp Med

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Authors - Miao Liu
  • KD Validated
mouseWB

Autophagy

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.

Authors - Jingru Bai
humanWB

Proc Natl Acad Sci U S A

Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.

Authors - Liu Wei W
mouseWB

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
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