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验证数据展示

  • WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

    WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

    IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIM2 antibody included
  • IP experiment of Raji using 20590-1-AP (same clone as 20590-1-PBS)

    IP experiment of Raji using 20590-1-AP (same clone as 20590-1-PBS)

    IP result of anti-AIM2 (IP:20590-1-AP, 4ug; Detection:20590-1-AP 1:600) with Raji cells lysate 2200 ug. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

    WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 20590-1-AP (same clone as 20590-1-PBS)

    IF Staining of MCF-7 using 20590-1-AP (same clone as 20590-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AIM2 Polyclonal antibody, PBS Only

AIM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20590-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

Interferon-inducible protein AIM2, PYHIN4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

产品信息

20590-1-PBS targets AIM2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14520

Product name: Recombinant human AIM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-343 aa of BC010940

Sequence: MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 absent in melanoma 2
别名 Interferon-inducible protein AIM2, PYHIN4
计算分子量 343 aa, 39 kDa
观测分子量 39-42 kDa
GenBank蛋白编号BC010940
基因名称 AIM2
Gene ID (NCBI) 9447
RRIDAB_10694420
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14862
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria (PMID:22695634). Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity (PMID:16432157,20566831). It has been reported that AIM2 protein levels are significantly elevated in alveolar macrophages of patients with IPF and bleomycin-treated mice (PMID: 38078661). AIM2 inflammasome was also significantly activated, which was also confirmed in Aβ-treated microglial cells (PMID: 34861878)

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