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验证数据展示

  • WB analysis using 14778-1-AP (same clone as 14778-1-PBS)

    WB analysis using 14778-1-AP (same clone as 14778-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14778-1-AP (AIFM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

AIFM3 Polyclonal antibody, PBS Only

AIFM3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14778-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EC:1.-.-.-, Apoptosis-inducing factor-like protein, Apoptosis-inducing factor 3, Apoptosis inducing factor 3, AIFL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14778-1-PBS targets AIFM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6278

Product name: Recombinant human AIFM3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 355-598 aa of BC032485

Sequence: AHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVETGDMSWLTGKGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptosis-inducing factor, mitochondrion-associated, 3
别名 EC:1.-.-.-, Apoptosis-inducing factor-like protein, Apoptosis-inducing factor 3, Apoptosis inducing factor 3, AIFL
计算分子量 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC032485
基因名称 AIFM3
Gene ID (NCBI) 150209
RRIDAB_2258090
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NN9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is a mitochondrial protein/flavoenzyme and its gene is located on chromosome 22q11.21. Mature human AIFM3 protein consists of 598 amino acids with a molecular weight of 66 kDa and is predominantly localized in the mitochondria. AIFM3 is widely expressed in many tissues, and breast cancer tissues and cholangiocarcinoma tissue (PMID: 32664187).

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