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验证数据展示

  • WB analysis using 17522-1-AP

    WB analysis using 17522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17522-1-AP (Actin-cardiac-muscle-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Alpha cardiac muscle actin specific Polyclonal antibody

Alpha cardiac muscle actin specific Polyclonal Antibody for WB, ELISA
Cat No. 17522-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Actin-cardiac-muscle-1-Specific, ACTC1, Alpha actin, ACTC, ACTC 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17522-1-AP targets Alpha cardiac muscle actin specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, alpha, cardiac muscle 1
别名 Actin-cardiac-muscle-1-Specific, ACTC1, Alpha actin, ACTC, ACTC 1
计算分子量 42 kDa
观测分子量42 kDa
GenBank蛋白编号NM_005159
基因名称 ACTC1
Gene ID (NCBI) 70
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68032
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

实验方案

Product Specific Protocols
WB protocol for Alpha cardiac muscle actin specific antibody 17522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols