Beta Actin Polyclonal antibody

beta actin Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10230-1-AP

Print datasheet

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



发表文章中的应用

WBSee 9 publications below

产品信息

10230-1-AP targets beta actin in WB, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, bovine
Immunogen beta actin fusion protein Ag0297 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, beta
Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
Calculated Molecular Weight 375 aa, 42 kDa
GenBank Accession NumberBC002409
Gene Symbol ACTB
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60709
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Beta Actin has been widely used as the internal control in bioscience. At least six isoforms are known in mammals. ALPHA-actin was predominant in single cells and BETA-actin was major in the cultured cells. Nonmuscle BETA- and GAMMA-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells. ALPHA-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, ALPHA- and GAMMA-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. This antibody can detect endogenous level of β-Actin and may cross-react with other isoforms of actin family.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

发表文章

SpeciesApplicationTitle
humanWB

Int J Nanomedicine

Integration of Dual Targeting and Dual Therapeutic Modules Endows Self-Assembled Nanoparticles with Anti-Tumor Growth and Metastasis Functions.

Authors - Biao Chen
humanWB

Eur J Pharmacol

Suppression of hypoxia-induced CAV1 autophagic degradation enhances nanoalbumin-paclitaxel transcytosis and improves therapeutic activity in pancreatic cancer

Authors - Xiangli Bai
humanWB

J Cell Biochem

Dependence of artesunate on long noncoding RNA-RP11 to inhibit epithelial-mesenchymal transition of hepatocellular carcinoma.

Authors - Wu Jing
bovineWB

Biol Trace Elem Res

The Protective Effect of Selenium on Bovine Mammary Epithelial Cell Injury Caused by Depression of Thioredoxin Reductase.

Authors - Yongmei Guo
humanWB

BMC Urol

Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway.

Authors - Cai Lv
WB

Int J Mol Med

miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells.

Authors - Tao Han