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验证数据展示

  • IF Staining of HepG2 using 22862-1-AP (same clone as 22862-1-PBS)

    IF Staining of HepG2 using 22862-1-AP (same clone as 22862-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22862-1-AP (ACSM2A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • WB analysis using 22862-1-AP (same clone as 22862-1-PBS)

    WB analysis using 22862-1-AP (same clone as 22862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

    IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

    IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

ACSM2A Polyclonal antibody, PBS Only

ACSM2A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22862-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

22862-1-PBS targets ACSM2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18856

Product name: Recombinant human ACSM2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 223-390 aa of BC125176

Sequence: GTSGLPKMAEHSYSSLGLKAKMDAGWTGLQASDIMWTISDTGWILNILCSLMEPWALGACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLLQQDLSSYKFPHLQNCVTVGESLLPETLENWRAQTGLDIRESYGQTETGLTCMVSKTMKIKPGYMGTAASCY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase medium-chain family member 2A
别名
计算分子量 577 aa, 64 kDa
观测分子量 60-64 kDa
GenBank蛋白编号BC125176
基因名称 ACSM2A
Gene ID (NCBI) 123876
RRIDAB_2879181
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08AH3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSM2A(Acyl-coenzyme A synthetase ACSM2A, mitochondrial) is also named as ACSM2, MACS2 and belongs to the ATP-dependent AMP-binding enzyme family. It acts as a medium-chain fatty acid : CoA ligase with the caculated molecular weight of 64 kDa and acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids.

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