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验证数据展示

  • WB analysis of HEK-293 using 20710-1-AP (same clone as 20710-1-PBS)

    WB analysis of HEK-293 using 20710-1-AP (same clone as 20710-1-PBS)

    WB result of ACSL3 antibody (20710-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

    WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

    WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 20710-1-AP (same clone as 20710-1-PBS)

    IHC staining of human stomach cancer using 20710-1-AP (same clone as 20710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20710-1-AP (ACSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL3 antibody included
  • Featured Product
  • KD/KO Validated

ACSL3 Polyclonal antibody, PBS Only

ACSL3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20710-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

ACS3, ACSL 3, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ACSL3 Ready-To-Use IHC Kit. ACSL3 primary antibody included.

产品信息

20710-1-PBS targets ACSL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 3
别名 ACS3, ACSL 3, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.2
计算分子量 80 kDa
观测分子量 70-80 kDa
GenBank蛋白编号NM_004457
基因名称 ACSL3
Gene ID (NCBI) 2181
RRIDAB_10697670
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95573
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

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